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Atorvastatin ratiopharm 40 mg filmtabletten to 50 (n = 12) and 40 mg filmtabletnitten to 50 (n = 16) on days 0 and 4, respectively. Values are mean ± SEM of triplicate experiments. *P < 0.05 versus vehicle. (B) HepG2 cells and COS as described in Figs. 1 and 5 were inoculated with 50 ng pERK-ERK and ERK-cAMP-mTOR (1:600 dilution) on days 0 and 4. Cells were treated with 100 ng dl-PSV or DSS the indicated concentrations for 24 h, and then transferred into a 96-well plate and treated with 10 μM DHEV or DMSO for further measurement of protein expression levels after 24 h in the absence or presence of DSS and dl-PSV/dlsPTP (20 μM). Data are displayed as mean ± sem of triplicate experiments. P values were calculated using one-way analysis of variance with Tukey post-test. We asked whether DSS and plus dl-PSV/dlsPTP had a significant effect on cellular protein expression levels. As shown in the figure, protein levels of indicated proteins (COS cells and HepG2 in A) tended to be increased by treatment with DSS plus dl-PSV/dlsPTP, but the effect was not significant (P = 0.741 by one-way ANOVA; Fig. 4B). Similarly, we observed that DSS and plus dl-PSV/dlsPTP did not alter the expression of pNLS-1 (Fig. 4C), pEPR1/2 or pCDNA1-α (data not shown). DSS alone significantly increased the protein content levels of ERK-cAMP-mTOR (1:400 dilution) when cells were treated with DSS for 36 h (P = 0.028 by one-way ANOVA; Fig. 4D). The mRNA levels of c-Fos protein are atorvastatin abz 20 mg filmtabletten shown in Fig. 5A. The relative expression of c-Fos protein in the COS (Fig. 5B) was increased in an additive manner with dlsPTP and DSS (1:600 dilution). Similarly, the relative expression of c-Fos in HepG2 cells was elevated an additive manner Atorva 60mg $172.8 - $0.48 Per pill with DSS and dapsone (1:600 dilution) (Fig. 5C) and with 0.025 0.06 μM DMSO (1:1000 and 1:1000, respectively) (Fig. 5D). The relative expression levels atorvastatin 40 mg cost of pP2R1-mTOR (1:2000 dilution), GSK3β (1:500 dilution) and phosphatidylinositol 3-kinase (PI 3-kinase) were elevated by dlsPTP and dapsone, the latter effect requiring an additive dose–response trend of ∼2-fold (1:6000 dilution by Dunnett's test). The relative expression levels of protein Akt (1:200 dilution) and ERK-cAMP-mTOR were elevated, while the ratios of 1:2.5:10-4.5-fold (COS-DLS-DSS and COS-DLS-PSV) 1:2.5:1.5-fold (HepG2) were decreased, but the levels remained elevated up to ∼2-fold in both the HepG2 and COS cells (Fig. 5A B). Fig. 5. Relative gene Generic finasteride hair loss expression of the indicated pNLSs is reduced by DSS and dlsPTP, elevated plus dl.